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total internal reflection fluorescence/spinning disk confocal microscopy carl zeiss tirf 3/cell observer sd  (Carl Zeiss)


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    Carl Zeiss total internal reflection fluorescence/spinning disk confocal microscopy carl zeiss tirf 3/cell observer sd
    Total Internal Reflection Fluorescence/Spinning Disk Confocal Microscopy Carl Zeiss Tirf 3/Cell Observer Sd, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/spinning+disk+(sd)+microscopy/1+elyra+microscope+ps/pm39844613-76-8-15
    Average 90 stars, based on 1 article reviews
    total internal reflection fluorescence/spinning disk confocal microscopy carl zeiss tirf 3/cell observer sd - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: Role of sorting nexin PaATG24 in autophagy, aging and development of Podospora anserina.
    Article Snippet: Microscopic analysis was performed with Zeiss Cell Observer SD – Spinning Disk Confocal Microscope (Carl Zeiss Microscopy, Jena, Germany) with a 63x/1.4 oil objective lens (Carl Zeiss Microscopy, Jena, Germany).

    Article Title: Effects o follicular fluid oxidative status on human mural granulosa cells, oocyte competency and ICSI parameters.
    Article Snippet: Abstract Purpose The aim of the present study was to understand the molecular and genetic alterations involved in follicular fluid oxidative process by investigating human mural granulosa cells and to find possible biomarkers for oocyte competency and ICSI outcome measures.. Methods: A total of 166 patients were included in the study.. Total antioxidant and oxidant levels of follicular fluids were measured on the day of oocyte pick-up and oxidative status were calculated.

    Article Title: Differential Early in vivo Dynamics and Functionality of Recruited Polymorphonuclear Neutrophils After Infection by Planktonic or Biofilm Staphylococcus aureus
    Article Snippet: Briefly, the inner side of the ear pinna was flattened on a glass slide and imaged on the ZEISS Spinning Disk Cell Observer (SD) (Carl Zeiss Microscopy) confocal microscope.

    Article Title: Learning-induced plasticity in the barrel cortex is disrupted by inhibition of layer 4 somatostatin-containing interneurons.
    Article Snippet: The immunofluorescence signal was analyzed using confocal or fluorescence microscopy (Zeiss Cell Observer SD Spinning Disk Confocal Microscope or Nikon Eclipse 80i, respectively).

    Staining:

    Article Title: Disruption of amygdala Tsc2 in adolescence leads to changed prelimbic cellular activity and generalized fear responses at adulthood in rats.
    Article Snippet: The following primary and secondary antibodies were used: rabbit anti-Tuberin/TSC2 (D93F12; Cell Signaling Technology, Danvers, MA, Cat. No. 4308), polyclonal chicken anti-MAP2 (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No. PA1-10005). .. Stained in vitro cultures of primary hippocampal neurons were documented by means of spinning disk confocal fluorescence microscopy (Cell Observer SD equipped with a 63× Plan-Apochromat oil immersion objective, Carl Zeiss Microscopy GmbH). ..

    In Vitro:

    Article Title: Disruption of amygdala Tsc2 in adolescence leads to changed prelimbic cellular activity and generalized fear responses at adulthood in rats.
    Article Snippet: The following primary and secondary antibodies were used: rabbit anti-Tuberin/TSC2 (D93F12; Cell Signaling Technology, Danvers, MA, Cat. No. 4308), polyclonal chicken anti-MAP2 (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No. PA1-10005). .. Stained in vitro cultures of primary hippocampal neurons were documented by means of spinning disk confocal fluorescence microscopy (Cell Observer SD equipped with a 63× Plan-Apochromat oil immersion objective, Carl Zeiss Microscopy GmbH). ..

    Fluorescence:

    Article Title: Disruption of amygdala Tsc2 in adolescence leads to changed prelimbic cellular activity and generalized fear responses at adulthood in rats.
    Article Snippet: The following primary and secondary antibodies were used: rabbit anti-Tuberin/TSC2 (D93F12; Cell Signaling Technology, Danvers, MA, Cat. No. 4308), polyclonal chicken anti-MAP2 (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No. PA1-10005). .. Stained in vitro cultures of primary hippocampal neurons were documented by means of spinning disk confocal fluorescence microscopy (Cell Observer SD equipped with a 63× Plan-Apochromat oil immersion objective, Carl Zeiss Microscopy GmbH). ..

    Microscopy:

    Article Title: Disruption of amygdala Tsc2 in adolescence leads to changed prelimbic cellular activity and generalized fear responses at adulthood in rats.
    Article Snippet: The following primary and secondary antibodies were used: rabbit anti-Tuberin/TSC2 (D93F12; Cell Signaling Technology, Danvers, MA, Cat. No. 4308), polyclonal chicken anti-MAP2 (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No. PA1-10005). .. Stained in vitro cultures of primary hippocampal neurons were documented by means of spinning disk confocal fluorescence microscopy (Cell Observer SD equipped with a 63× Plan-Apochromat oil immersion objective, Carl Zeiss Microscopy GmbH). ..

    Article Title: Super-resolution imaging illuminates new dynamic behaviors of cellulose synthase.
    Article Snippet: SIM (3D SIM) and near-TIRF imaging were performed on a Nikon N-SIM/N-STORM microscope using a 100 1.49 NA oil-immersion objective and an Andor back-thinned iXON 897 EMCCD camera (512 512 pixels). .. Confocal microscopy was performed on a Zeiss Observer SD spinning disk confocal microscope with a 100 1.40 NA oil-immersion objective and a Photometrics QuantEM 512SC camera (512 512 pixels). ..

    Article Title: 3D in vitro culture system to study collective migration in mammary organoid epithelium
    Article Snippet: .. For time-lapse imaging, DIC microscopy can be performed on a Zeiss Cell Observer SD spinning disk confocal microscope using an EC Plan-Neofluar 10×/0.3 Ph1 M27 objective lens (Item no.: 420341-9911-000), an Analy DIC Transmission light reflector, an sCMOS camera at 37°C with 5% CO 2 . ..

    Article Title: Mitochondrial Metabolism Drives Low-density Lipoprotein-induced Breast Cancer Cell Migration
    Article Snippet: For zebrafish xenografts imaging and analysis, initial screening was performed using an inverted fluorescence widefield microscope Zeiss Axiovert 200M, with 10× amplification. .. For the tropism evaluation, tiles of zebrafish larvae were acquired using the spinning disk confocal microscope Zeiss Cell Observer SD and quantified with ImageJ (SCR_003070, cell counter plugin). .. For the quantification of cells per organ, Imaris (SCR_007370) was used.

    Confocal Microscopy:

    Article Title: Super-resolution imaging illuminates new dynamic behaviors of cellulose synthase.
    Article Snippet: SIM (3D SIM) and near-TIRF imaging were performed on a Nikon N-SIM/N-STORM microscope using a 100 1.49 NA oil-immersion objective and an Andor back-thinned iXON 897 EMCCD camera (512 512 pixels). .. Confocal microscopy was performed on a Zeiss Observer SD spinning disk confocal microscope with a 100 1.40 NA oil-immersion objective and a Photometrics QuantEM 512SC camera (512 512 pixels). ..

    Imaging:

    Article Title: 3D in vitro culture system to study collective migration in mammary organoid epithelium
    Article Snippet: .. For time-lapse imaging, DIC microscopy can be performed on a Zeiss Cell Observer SD spinning disk confocal microscope using an EC Plan-Neofluar 10×/0.3 Ph1 M27 objective lens (Item no.: 420341-9911-000), an Analy DIC Transmission light reflector, an sCMOS camera at 37°C with 5% CO 2 . ..

    Transmission Assay:

    Article Title: 3D in vitro culture system to study collective migration in mammary organoid epithelium
    Article Snippet: .. For time-lapse imaging, DIC microscopy can be performed on a Zeiss Cell Observer SD spinning disk confocal microscope using an EC Plan-Neofluar 10×/0.3 Ph1 M27 objective lens (Item no.: 420341-9911-000), an Analy DIC Transmission light reflector, an sCMOS camera at 37°C with 5% CO 2 . ..



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    Targeting C1GALT1 promotes doxorubicin‐induced apoptosis while impairing doxorubicin efflux. (A) Immunoblotting analysis for C1GALT1 expression in various OS cell lines, using GAPDH as an internal control. (B) RT‐qPCR analysis showing mRNA expression levels of C1GALT1 in SaOS‐2 and HOS cells stimulated with 50 n m doxorubicin for 48 h, using ACTB as an internal control. Immunoblotting analysis for C1GALT1 and GAPDH protein expression levels in the same treated cells. (C) Immunoblotting analysis of C1GALT1 expression in SaOS‐2 and HOS OS cell lines transfected with C1GALT1 shRNA or treated with itraconazole (2.5 μ m ), using GAPDH as an internal control. (D) Representative flow cytometry plots showing percentages of apoptotic cells in doxorubicin‐treated cells transfected with C1GALT1 shRNA or scramble control. Overlay histogram plots are shown on the right‐hand side of the panel. (E) Representative <t>fluorescence</t> images taken by TIRF/confocal microscope display residual intensity of doxorubicin (red) in cells over 1 h. Scale bar, 50 μm. * p < 0.05, **** p < 0.0001. Bars represent mean fluorescence intensity changes of randomly selected cells ( n = 30).
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    Targeting C1GALT1 promotes doxorubicin‐induced apoptosis while impairing doxorubicin efflux. (A) Immunoblotting analysis for C1GALT1 expression in various OS cell lines, using GAPDH as an internal control. (B) RT‐qPCR analysis showing mRNA expression levels of C1GALT1 in SaOS‐2 and HOS cells stimulated with 50 n m doxorubicin for 48 h, using ACTB as an internal control. Immunoblotting analysis for C1GALT1 and GAPDH protein expression levels in the same treated cells. (C) Immunoblotting analysis of C1GALT1 expression in SaOS‐2 and HOS OS cell lines transfected with C1GALT1 shRNA or treated with itraconazole (2.5 μ m ), using GAPDH as an internal control. (D) Representative flow cytometry plots showing percentages of apoptotic cells in doxorubicin‐treated cells transfected with C1GALT1 shRNA or scramble control. Overlay histogram plots are shown on the right‐hand side of the panel. (E) Representative <t>fluorescence</t> images taken by TIRF/confocal microscope display residual intensity of doxorubicin (red) in cells over 1 h. Scale bar, 50 μm. * p < 0.05, **** p < 0.0001. Bars represent mean fluorescence intensity changes of randomly selected cells ( n = 30).
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    Image Search Results


    Targeting C1GALT1 promotes doxorubicin‐induced apoptosis while impairing doxorubicin efflux. (A) Immunoblotting analysis for C1GALT1 expression in various OS cell lines, using GAPDH as an internal control. (B) RT‐qPCR analysis showing mRNA expression levels of C1GALT1 in SaOS‐2 and HOS cells stimulated with 50 n m doxorubicin for 48 h, using ACTB as an internal control. Immunoblotting analysis for C1GALT1 and GAPDH protein expression levels in the same treated cells. (C) Immunoblotting analysis of C1GALT1 expression in SaOS‐2 and HOS OS cell lines transfected with C1GALT1 shRNA or treated with itraconazole (2.5 μ m ), using GAPDH as an internal control. (D) Representative flow cytometry plots showing percentages of apoptotic cells in doxorubicin‐treated cells transfected with C1GALT1 shRNA or scramble control. Overlay histogram plots are shown on the right‐hand side of the panel. (E) Representative fluorescence images taken by TIRF/confocal microscope display residual intensity of doxorubicin (red) in cells over 1 h. Scale bar, 50 μm. * p < 0.05, **** p < 0.0001. Bars represent mean fluorescence intensity changes of randomly selected cells ( n = 30).

    Journal: The Journal of Pathology

    Article Title: C1GALT1 expression predicts poor survival in osteosarcoma and is crucial for ABCC1 transporter‐mediated doxorubicin resistance

    doi: 10.1002/path.6384

    Figure Lengend Snippet: Targeting C1GALT1 promotes doxorubicin‐induced apoptosis while impairing doxorubicin efflux. (A) Immunoblotting analysis for C1GALT1 expression in various OS cell lines, using GAPDH as an internal control. (B) RT‐qPCR analysis showing mRNA expression levels of C1GALT1 in SaOS‐2 and HOS cells stimulated with 50 n m doxorubicin for 48 h, using ACTB as an internal control. Immunoblotting analysis for C1GALT1 and GAPDH protein expression levels in the same treated cells. (C) Immunoblotting analysis of C1GALT1 expression in SaOS‐2 and HOS OS cell lines transfected with C1GALT1 shRNA or treated with itraconazole (2.5 μ m ), using GAPDH as an internal control. (D) Representative flow cytometry plots showing percentages of apoptotic cells in doxorubicin‐treated cells transfected with C1GALT1 shRNA or scramble control. Overlay histogram plots are shown on the right‐hand side of the panel. (E) Representative fluorescence images taken by TIRF/confocal microscope display residual intensity of doxorubicin (red) in cells over 1 h. Scale bar, 50 μm. * p < 0.05, **** p < 0.0001. Bars represent mean fluorescence intensity changes of randomly selected cells ( n = 30).

    Article Snippet: Doxorubicin autofluorescence (Ex/Em = 488/600 nm) was monitored using total internal reflection fluorescence (TIRF)/spinning disk confocal microscopy (Carl Zeiss, TIRF 3/Cell Observer SD; Imaging Core, First Core Labs, NTU College of Medicine).

    Techniques: Western Blot, Expressing, Control, Quantitative RT-PCR, Transfection, shRNA, Flow Cytometry, Fluorescence, Microscopy

    ITZ mimics the effects of boosting doxorubicin‐induced apoptosis and reducing doxorubicin efflux. (A) Representative flow cytometry plots showing percentages of apoptotic cells in doxorubicin (50 n m ) or ITZ) (2.5 μ m )‐treated OS cells. (B and C) Representative fluorescence images taken by TIRF/confocal microscope display residual intensity of doxorubicin (red) in cells over 1 h. Scale bar, 50 μm. **** p < 0.0001. Bars represent the mean fluorescence intensity changes of randomly selected cells ( n = 30).

    Journal: The Journal of Pathology

    Article Title: C1GALT1 expression predicts poor survival in osteosarcoma and is crucial for ABCC1 transporter‐mediated doxorubicin resistance

    doi: 10.1002/path.6384

    Figure Lengend Snippet: ITZ mimics the effects of boosting doxorubicin‐induced apoptosis and reducing doxorubicin efflux. (A) Representative flow cytometry plots showing percentages of apoptotic cells in doxorubicin (50 n m ) or ITZ) (2.5 μ m )‐treated OS cells. (B and C) Representative fluorescence images taken by TIRF/confocal microscope display residual intensity of doxorubicin (red) in cells over 1 h. Scale bar, 50 μm. **** p < 0.0001. Bars represent the mean fluorescence intensity changes of randomly selected cells ( n = 30).

    Article Snippet: Doxorubicin autofluorescence (Ex/Em = 488/600 nm) was monitored using total internal reflection fluorescence (TIRF)/spinning disk confocal microscopy (Carl Zeiss, TIRF 3/Cell Observer SD; Imaging Core, First Core Labs, NTU College of Medicine).

    Techniques: Flow Cytometry, Fluorescence, Microscopy

    ABCC1 identification in doxorubicin resistance and efflux and clinical correlation to C1GALT1 in OS. (A) Bar charts depict ABC transporter gene expression using the TaqMan™ Human ABC Transporter Array in doxorubicin‐selected HOS and SaOS‐2 cell lines, covering 50 genes across seven ABC transporter families. Arrows indicate the relatively high expression of ABC transporters, with GAPDH used as an internal control. (B) Representative flow cytometry plots show percentages of apoptotic cells in doxorubicin‐treated cells transfected with corresponding ABC shRNA or scramble control. (C and D) Representative fluorescence images captured by TIRF/confocal microscopy demonstrate residual intensity of doxorubicin (red) in cells over 1 h. Scale bar, 50 μm. Bars represent mean fluorescence intensity changes of randomly selected cells ( n = 30). **** p < 0.0001. (E) Representative IHC images display C1GALT1 and ABCC1 protein staining in OS tumors with and without recurrence. Brown color indicates positive staining. Scale bars, 100 μm; high‐magnification images of marked area are shown in lower right corner. (F) Kaplan–Meier curves depict overall survival and PFS according to ABCC1 expression in 29 OS patients ( p = 0.0135 and 0.0086, respectively, log‐rank test). (G) Pearson correlation analysis of C1GALT1 and ABCC1 protein expression in the same patient cohort ( r = 0.45, p < 0.05).

    Journal: The Journal of Pathology

    Article Title: C1GALT1 expression predicts poor survival in osteosarcoma and is crucial for ABCC1 transporter‐mediated doxorubicin resistance

    doi: 10.1002/path.6384

    Figure Lengend Snippet: ABCC1 identification in doxorubicin resistance and efflux and clinical correlation to C1GALT1 in OS. (A) Bar charts depict ABC transporter gene expression using the TaqMan™ Human ABC Transporter Array in doxorubicin‐selected HOS and SaOS‐2 cell lines, covering 50 genes across seven ABC transporter families. Arrows indicate the relatively high expression of ABC transporters, with GAPDH used as an internal control. (B) Representative flow cytometry plots show percentages of apoptotic cells in doxorubicin‐treated cells transfected with corresponding ABC shRNA or scramble control. (C and D) Representative fluorescence images captured by TIRF/confocal microscopy demonstrate residual intensity of doxorubicin (red) in cells over 1 h. Scale bar, 50 μm. Bars represent mean fluorescence intensity changes of randomly selected cells ( n = 30). **** p < 0.0001. (E) Representative IHC images display C1GALT1 and ABCC1 protein staining in OS tumors with and without recurrence. Brown color indicates positive staining. Scale bars, 100 μm; high‐magnification images of marked area are shown in lower right corner. (F) Kaplan–Meier curves depict overall survival and PFS according to ABCC1 expression in 29 OS patients ( p = 0.0135 and 0.0086, respectively, log‐rank test). (G) Pearson correlation analysis of C1GALT1 and ABCC1 protein expression in the same patient cohort ( r = 0.45, p < 0.05).

    Article Snippet: Doxorubicin autofluorescence (Ex/Em = 488/600 nm) was monitored using total internal reflection fluorescence (TIRF)/spinning disk confocal microscopy (Carl Zeiss, TIRF 3/Cell Observer SD; Imaging Core, First Core Labs, NTU College of Medicine).

    Techniques: Gene Expression, Expressing, Control, Flow Cytometry, Transfection, shRNA, Fluorescence, Confocal Microscopy, Staining

    ABCC1 is involved in the phenotypic changes mediated by C1GALT1 in OS cells both in vitro and in vivo . (A) Fluorescence images captured using TIRF/confocal microscopy display the residual intensity of doxorubicin (red) in mock, C1GALT1‐overexpressing, and C1GALT1‐overexpressing with ABCC1‐silenced cells over 1 h. Scale bar, 50 μm. Bars represent mean fluorescence intensity changes of randomly selected cells ( n = 30). **** p < 0.0001. (B) Representative flow cytometry plots display percentages of apoptotic cells in doxorubicin‐treated samples. (C) IVIS images of representative mice taken at 2‐week intervals, highlighting the regions of interest (ROIs, marked by ovals) around tibial tumors in mice injected with either G292 mock‐transfected cells (black bar and circles), C1GALT1‐overexpressing cells (ovC1GALT1, green bar and squares), or C1GALT1 ‐overexpressing cells with ABCC1 knockdown (ovC1GALT1/shABCC1, purple bar and triangles). H&E‐stained sections of tibial tumors are shown on the right. (D) Bars represent mean bioluminescent signal intensity within tumor ROIs ( n = 6). ** p < 0.01. Tumor growth curves show tumor volume (mm 3 ). (E) Tumor volumes were measured by calipers at 0, 14, and 28 days after injection. p < 0.01 at 28 days, indicating significant differences between ovC1GALT1 group and both the mock and ovC1GALT1/shABCC1 groups.

    Journal: The Journal of Pathology

    Article Title: C1GALT1 expression predicts poor survival in osteosarcoma and is crucial for ABCC1 transporter‐mediated doxorubicin resistance

    doi: 10.1002/path.6384

    Figure Lengend Snippet: ABCC1 is involved in the phenotypic changes mediated by C1GALT1 in OS cells both in vitro and in vivo . (A) Fluorescence images captured using TIRF/confocal microscopy display the residual intensity of doxorubicin (red) in mock, C1GALT1‐overexpressing, and C1GALT1‐overexpressing with ABCC1‐silenced cells over 1 h. Scale bar, 50 μm. Bars represent mean fluorescence intensity changes of randomly selected cells ( n = 30). **** p < 0.0001. (B) Representative flow cytometry plots display percentages of apoptotic cells in doxorubicin‐treated samples. (C) IVIS images of representative mice taken at 2‐week intervals, highlighting the regions of interest (ROIs, marked by ovals) around tibial tumors in mice injected with either G292 mock‐transfected cells (black bar and circles), C1GALT1‐overexpressing cells (ovC1GALT1, green bar and squares), or C1GALT1 ‐overexpressing cells with ABCC1 knockdown (ovC1GALT1/shABCC1, purple bar and triangles). H&E‐stained sections of tibial tumors are shown on the right. (D) Bars represent mean bioluminescent signal intensity within tumor ROIs ( n = 6). ** p < 0.01. Tumor growth curves show tumor volume (mm 3 ). (E) Tumor volumes were measured by calipers at 0, 14, and 28 days after injection. p < 0.01 at 28 days, indicating significant differences between ovC1GALT1 group and both the mock and ovC1GALT1/shABCC1 groups.

    Article Snippet: Doxorubicin autofluorescence (Ex/Em = 488/600 nm) was monitored using total internal reflection fluorescence (TIRF)/spinning disk confocal microscopy (Carl Zeiss, TIRF 3/Cell Observer SD; Imaging Core, First Core Labs, NTU College of Medicine).

    Techniques: In Vitro, In Vivo, Fluorescence, Confocal Microscopy, Flow Cytometry, Injection, Transfection, Knockdown, Staining

    O‐glycosylation changes induced by silencing C1GALT1 hinder ABCC1 cell‐surface targeting and promote its lysosomal degradation. (A) Histograms showing VVA lectin binding fluorescence intensity in mock, C1GALT1‐silenced, and itraconazole‐treated cells. HOS cells were stained with FITC‐conjugated VVA lectin or without lectin (n.c.). (B) Western blot showing proteins pulled down (PD) by VVA lectin in mock, C1GALT1‐silenced, and itraconazole‐treated cells, then immunoblotted (IB) with anti‐ABCC1. GAPDH was the internal control. (C) Western blot demonstrating ABCC1 expression in HOS cells treated with CQ and MG132 inhibitors (10 μ m each). (D) Immunofluorescence staining of mock, C1GALT1‐silenced, and itraconazole‐treated cells for ABCC1 (green) and GM130 (red). Colocalization at the Golgi apparatus is shown in yellow in the merged image of C1GALT1‐silenced samples. (E) Representative image showing tumors from paratibial injection of HOS cells in mice. Blue arrowheads indicate mock group; red arrowheads indicate the C1GALT1 knockdown group. Tumor volume, calculated as (length × width 2 )/2, is plotted over time ( n = 6). * p < 0.05, ** p < 0.01. (F) IHC images showing ABCC1 staining in tibia tumors. Brown indicates positive staining. Scale bars, 100 μm. High‐magnification images of marked areas are in lower right corner.

    Journal: The Journal of Pathology

    Article Title: C1GALT1 expression predicts poor survival in osteosarcoma and is crucial for ABCC1 transporter‐mediated doxorubicin resistance

    doi: 10.1002/path.6384

    Figure Lengend Snippet: O‐glycosylation changes induced by silencing C1GALT1 hinder ABCC1 cell‐surface targeting and promote its lysosomal degradation. (A) Histograms showing VVA lectin binding fluorescence intensity in mock, C1GALT1‐silenced, and itraconazole‐treated cells. HOS cells were stained with FITC‐conjugated VVA lectin or without lectin (n.c.). (B) Western blot showing proteins pulled down (PD) by VVA lectin in mock, C1GALT1‐silenced, and itraconazole‐treated cells, then immunoblotted (IB) with anti‐ABCC1. GAPDH was the internal control. (C) Western blot demonstrating ABCC1 expression in HOS cells treated with CQ and MG132 inhibitors (10 μ m each). (D) Immunofluorescence staining of mock, C1GALT1‐silenced, and itraconazole‐treated cells for ABCC1 (green) and GM130 (red). Colocalization at the Golgi apparatus is shown in yellow in the merged image of C1GALT1‐silenced samples. (E) Representative image showing tumors from paratibial injection of HOS cells in mice. Blue arrowheads indicate mock group; red arrowheads indicate the C1GALT1 knockdown group. Tumor volume, calculated as (length × width 2 )/2, is plotted over time ( n = 6). * p < 0.05, ** p < 0.01. (F) IHC images showing ABCC1 staining in tibia tumors. Brown indicates positive staining. Scale bars, 100 μm. High‐magnification images of marked areas are in lower right corner.

    Article Snippet: Doxorubicin autofluorescence (Ex/Em = 488/600 nm) was monitored using total internal reflection fluorescence (TIRF)/spinning disk confocal microscopy (Carl Zeiss, TIRF 3/Cell Observer SD; Imaging Core, First Core Labs, NTU College of Medicine).

    Techniques: Glycoproteomics, Binding Assay, Fluorescence, Staining, Western Blot, Control, Expressing, Immunofluorescence, Injection, Knockdown